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Immunology and Infection
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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

DOI:
10.3791/62324-v

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14:58 min

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March 05, 2022

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, , , , ,
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Chapters

  • 00:07Introduction
  • 01:15Docker Desktop Installation
  • 04:25Quality Control
  • 05:10Mapping and Annotation Steps
  • 05:51Differentially Expressed Genes Process
  • 06:10Transfer Data to the Personal Computer
  • 12:02Representative Results
  • 14:22Conclusions

Summary

Automatic Translation

Automatic Translation

The protocol presented here describes a complete pipeline to analyze RNA-sequencing transcriptome data from raw reads to functional analysis, including quality control and preprocessing steps to advanced statistical analytical approaches.

Tags

– High-throughput Transcriptome Analysis- Host-pathogen Interactions- Quality Control- Sequencing And Annotations- Statistical And Co-expression Analysis- Molecular Degree Of Perturbation Analysis- Functional Analysis- Docker Container- Chikungunya Virus- RNA Sequencing- Reference Genome- Gene Annotation

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