Niigata University Graduate School of Medical and Dental Sciences 2 articles published in JoVE Biology Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1 Yuko Miyagawa1, Kazunori Nagasaka1, Kaoru Yamawaki2,3, Yutaro Mori2,3, Tatsuya Ishiguro2, Kei Hashimoto1, Ryoko Koike1, Siho Fukui1, Takeru Sugihara1, Takayuki Ichinose1, Haruko Hiraike1, Koichiro Kido1, Koji Okamoto3, Takayuki Enomoto2, Takuya Ayabe1 1Department of Obstetrics and Gynecology, Teikyo University School of Medicine, 2Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, 3Devision of Cancer Differentiation, National Cancer Center Research Institute Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro. Medicine Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy Eriko Katsuta1, Masanori Oshi1, Omar M. Rashid2,3,4,5, Kazuaki Takabe1,6,7,8,9,10 1Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, 2Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, 3Department of Surgery, Massachusetts General Hospital, 4Department of Surgery, University of Miami Miller School of Medicine, 5Department of Surgery, Nova Southeastern University School of Medicine, 6Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, 7Department of Breast Surgery and Oncology, Tokyo Medical University, 8Department of Surgery, Yokohama City University, 9Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, 10Department of Surgery, Fukushima Medical University We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.
