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Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

Article DOI: 10.3791/61751-v • 07:59 min • December 5th, 2020
December 5th, 2020 •
1, 1, 2,3, 2,3, 2, 1, 1, 1, 1, 1, 1, 1, 3, 2, 1
1Department of Obstetrics and Gynecology, Teikyo University School of Medicine, 2Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, 3Devision of Cancer Differentiation, National Cancer Center Research Institute

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Summary
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Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro.

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Angiogenetic Properties Ovarian Cancer Stem-like Cells Three-dimensional Co-culture System NICO-1 Mortality Rate Gynecological Malignancies Vascular Niche Tumor Microenvironment Reproducibility Tumor Initiation Capabilities Angiogenesis Yuko Miyagawa Research Fellow Laboratory Cancer Stem Cells Isolation Human Ovarian Cancer Ascites Paracentesis OCSC Medium Non-ionic Density Gradient Medium PBS Cell Suspensions Density Gradient Centrifugation Interphase 15 Milliliter Tube Wash Cells Fresh OCSC Medium
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