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Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
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Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

Article DOI: 10.3791/2645-v • 09:39 min • May 31st, 2011
May 31st, 2011 •
1, *1, *1, 1
1Department of Cell and Molecular Biology, Northwestern University
* These authors contributed equally

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This article details the procedures involved in overexpression and analysis of small GTPases in polarized epithelial cells using microinjection technique.

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Small GTPases Microinjection Plasmids Polarized Epithelial Cells Apical Domain Basolateral Domain Tight Junctions MDCK Cells Cell Polarity Ras Superfamily RalA Cdc42 Rab8 Rab10 Rab13 GDP-bound State GTP-bound State Nucleotide Binding Regions Dominant Negative Dominant Active Alleles Overexpression
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