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Journal / Medicine / Isolation, Culture, and Imaging of Human Fetal…
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Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
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Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

Article DOI: 10.3791/50796-v • 08:09 min • May 18th, 2014
May 18th, 2014 •
1, 1, 1, 1
1Pediatric Diabetes Research Center, Department of Pediatrics, University of California, San Diego

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A protocol to isolate, culture, and image islet cell clusters (ICCs) derived from human fetal pancreatic cells is described.  The method details the steps necessary to generate ICCs from tissue, culture as monolayers or in suspension as aggregates, and image for markers of proliferation and pancreatic cell fate decisions.

Tags

Isolation Culture Imaging Human Fetal Pancreatic Cell Clusters Kidney Capsule Transplantation Functioning Endocrine Cells Circulating Human C-peptide Glucose Stimulation Insulin Producing Cells Human Embryonic Stem Cells (hESC) Type 1 Diabetes Treatment Growth And Differentiation Of Endocrine Precursor Cells Protocols For Generating Insulin Producing Cells ICCs (islet Cell Clusters) Difficulty Of Working With Human Fetal Pancreas Gestational Ages Cell Proliferation Pancreatic Markers
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