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Immunology and Infection

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High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Article DOI: 10.3791/52195-v • 09:22 min • November 27th, 2014
November 27th, 2014 •
1,3, 1,2
1Department of Pharmacology, University of Minnesota, 2Department of Surgery, University of Minnesota, 33M Corporate Research Laboratory

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Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

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High Throughput Fluorometric Technique Assessment Macrophage Phagocytosis Actin Polymerization Fluorometric Analysis Cell Types Adherent Cells Non-adherent Cells Phagocytic Cell Types Macrophages Fluorescently Labeled Opsonized Particles Trypan Blue 96-well Plates Fluorescent Particles Phagocytose Varied Amounts Of Time Internalization Of Fluorescent Particles Trypan Blue Wash PBS Wash Fixation DAPI Staining Fluorometric Quantification Plate Reading Relative Fluorescence Units Phagocytic Index
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