Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Enza Lonardo; Michele Cioffi; Patricia Sancho; Shanthini Crusz; Christopher Heeschen

doi:10.3791/52801

Rivista

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Medicina

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Studiare cancro del pancreas staminali caratteristiche cellulari per lo sviluppo di nuove strategie di trattamento

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Studiare cancro del pancreas staminali caratteristiche cellulari per lo sviluppo di nuove strategie di trattamento

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19,507 Views

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07:29 min

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June 20, 2015

DOI:

10.3791/52801-v

DOI:

10.3791/52801-v

•

07:29 min

June 20, 2015

•

23 Views

Enza Lonardo^1,2, Michele Cioffi¹, Patricia Sancho^1,3, Shanthini Crusz³, Christopher Heeschen^1,3

¹Stem Cells & Cancer Group, Molecular Pathology Program,Spanish National Cancer Research Center, ²Institute for Research in Biomedicine (IRB Barcelona), ³Center for Stem Cells in Cancer & Ageing, Barts Cancer Institute,Queen Mary University of London

Summary

Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.