An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

Chun Liu; Christina Chan

doi:10.3791/54085

Rivista

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Neuroscienze

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Un approccio per migliorare l'allineamento e la mielinizzazione dorsale Root Ganglion neuroni

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

Un approccio per migliorare l'allineamento e la mielinizzazione dorsale Root Ganglion neuroni

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8,412 Views

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09:48 min

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August 24, 2016

DOI:

10.3791/54085-v

DOI:

10.3791/54085-v

•

09:48 min

August 24, 2016

•

3 Views

Chun Liu¹, Christina Chan^1,2

¹Department of Chemical Engineering and Materials Science,Michigan State University, ²Department of Biochemistry and Molecular Biology,Michigan State University

Trascrizione

Summary

This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.