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Detection of Copy Number Alterations Using Single Cell Sequencing
JoVE Journal
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JoVE Journal Genetica
Detection of Copy Number Alterations Using Single Cell Sequencing

Detection of Copy Number Alterations Using Single Cell Sequencing

DOI:
10.3791/55143-v

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09:45 min

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February 17, 2017

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, ,
1Koch Institute for Integrative Cancer Research, Department of Biology,Massachusetts Institute of Technology, 2Howard Hughes Medical Institute, 3Division of Health Sciences and Technology,Harvard Medical School, 4The Barbara K. Ostrom (1978) Bioinformatics and Computing Facility in the Swanson Biotechnology Center, Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 5BioMicro Center, Department of Biology,Massachusetts Institute of Technology

Capitoli

  • 00:05Titolo
  • 00:48Preparation of Aspirator
  • 04:34Whole Genome Amplification
  • 07:39Results: Detection of Copy Number Alterations Using Single Cell Sequencing
  • 08:48Conclusion

Summary

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Single cell sequencing is an increasingly popular and accessible tool for addressing genomic changes at high resolution. We provide a protocol that uses single cell sequencing to identify copy number alterations in single cells.

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Single Cell SequencingCopy Number AlterationsGenomic HeterogeneityCytologic ApproachesSensitivitySpecificityMicro-aspiratorSyringe FilterPVC TubingSerological PipetteGlass Capillary TubeWhole Genome AmplificationAdherence CellsTrypsinization10% Bleach70% Ethanol

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