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Cancer Research

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JoVE Journal Cancer Research
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Flow Cytometry-based Drug Screening-System für die Identifizierung von kleinen Molekülen, die zelluläre Differenzierung von Stammzellen Glioblastom zu fördern

Article DOI: 10.3791/56176-v • 10:28 min • January 10th, 2018
January 10th, 2018 •
1, 1, 2, 1,3, 1
1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine

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Eine effizientes Screening-Protokoll wird für die Identifikation von kleinen Molekülen präsentiert, die Astroglial Differenzierung in Glioblastom Stammzellen (vorliegenden Allgemeinen Geschäftsbedingungen) zu fördern. Der Test basiert auf Stammzell-Differenzierung Reporter, wobei der Ausdruck der verbesserte GFP (eGFP) durch den menschlichen GFAP Projektträger angetrieben wird.

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Krebsbiologie Ausgabe 131 Stammzellen Gliom Astrocytic Differenzierung Lentivirus Reporter System Drogentest Differenzierung Therapie Acetylcholin Kalzium-Signalisierung
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