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An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

Article DOI: 10.3791/57037-v • 06:39 min • September 12th, 2018
September 12th, 2018 •
1,2, 1,2, 1,2, 1,2,3
1Cardiovascular Pulmonary Research Laboratory, University of Colorado Denver, 2Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver, 3Denver VA Medical Center

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In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues.

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Optimized Evans Blue Protocol Vascular Leak Assessment Mouse Plasma Extravasation Leakage Modified Evans Blue Dye Method Endogenous Factors Exogenous Factors Catheterization Surgical Competence Venous Access Reproducibility Surgical Difficulty Catheter Placement Adult FVB NJ Mouse Toe Pinch Response Shave Ventral Neck Area Supine Position Preheated Pad Tape Paws Ointment For Eyes Incision In Right Ventral Neck Jugular Vein Lidocaine For Pain Management And Vasodilation Isolate Internal Jugular Vein Blunt Dissection Suture Tying
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