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Journal / Immunology and Infection / Imaging Ca2+ Responses During Shigella Infection…
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Immunology and Infection

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Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells

Article DOI: 10.3791/57728-v • 08:56 min • May 24th, 2018
May 24th, 2018 •
1,2,3,4, 1,2,3,4, 5, 1,2,3,4
1Equipe Communication Intercellulaire et Infections Microbiennes, Centre de Recherche Interdisciplinaire en Biologie (CIRB), Collège de France, 2Institut National de la Santé et de la Recherche Médicale (Inserm), U1050, 3Centre Nationale de la Recherche Scientifique (CNRS), UMR7241, 4MEMOLIFE Laboratory of Excellence and Paris Sciences et Lettres, 5Inserm, UMRS1174, Université Paris Sud

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Here, we present protocols to visualize calcium (Ca2+) responses elicited by HeLa cells infected by Shigella. By optimizing the parameters of bacterial infection and imaging with Ca2+ fluorescent probes, atypical global and local Ca2+ signals induced by bacteria over a large range of infection kinetics are characterized.

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Fluorescent Microscopy Imaging Ca2+ Responses Shigella Infection Epithelial Cells Chemical Probes Calcium Signals Host Cells Pathogenic Shigella Cellular Microbiology Cytoskeletal Reorganization Cell Death Technique Parameters Biological Samples Shigella Adhesion Congo Red TCS Agar Plate Pre-cultures Liquid Cultures Optical Density
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