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JoVE Journal Cancer Research
Aislamiento de células similares a tallos de cultivos esferoides tridimensionales
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Aislamiento de células similares a tallos de cultivos esferoides tridimensionales

Article DOI: 10.3791/60357-v • 09:06 min • December 13th, 2019
December 13th, 2019 •
1, 1, 1, 1, 1,2,3
1Department of Urology, College of Medicine, University of Illinois at Chicago, 2Department of Pathology, College of Medicine, University of Illinois at Chicago, 3University of Illinois Cancer Center, College of Medicine, University of Illinois at Chicago

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Utilizando células epiteliales de próstata primarias humanas, informamos de un nuevo método libre de biomarcadores de caracterización funcional de células similares a tallos mediante un ensayo de retención de etiquetas basado en esferoides. Se describe un protocolo paso a paso para el etiquetado de celdas 2D BrdU, CFSE o Far Red; formación de esferoides tridimensionales; identificación celular similar a un tallo que retiene la etiqueta por inmunocitoquímica; y aislamiento por FACS.

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Cancer Research Número 154 células madre células progenitoras aislamiento celular esferoides próstata muestras de cáncer
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