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Biochemistry

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Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Article DOI: 10.3791/63057-v • 07:24 min • September 23rd, 2021
September 23rd, 2021 •
1, 1
1Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, The University of Texas Health Science Center at San Antonio

Capitoli

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Automatic Translation

A protocol for the application of paramagnetic relaxation enhancement NMR spectroscopy to detect weak and transient inter- and intra-molecular interactions in intrinsically disordered proteins is presented.

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Paramagnetic Relaxation Enhancement Self-associations Intrinsically Disordered Proteins Intermolecular Distances Transient Biomolecular Interactions Lowly Populated Biomolecular Interactions PREs NMR Techniques Nitroxide Spin Label Spectra Analysis Pulse Calibration Protein Condensation 3-maleimido-PROXYL Stock Solution Isotopically Labeled Protein Incubation Room Temperature Protection From Light And Oxygen Gentle Rocking Or Nutation Non-specific Solvent PRE Dialysis Gel Filtration Ellman's Reagent Sulfhydryl Groups Absorbance Measurement
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