Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Balakrishnan S. Moorthy; Lavanya K. Iyer; Elizabeth M. Topp

doi:10.3791/52503

ジャーナル

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化学

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質量分析は、凍結乾燥粉末中のタンパク質の構造と相互作用を研究するためのアプローチ

JoVE Journal
化学

This content is Free Access.

JoVE Journal 化学

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

質量分析は、凍結乾燥粉末中のタンパク質の構造と相互作用を研究するためのアプローチ

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15,573 Views

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11:14 min

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April 14, 2015

DOI:

10.3791/52503-v

DOI:

10.3791/52503-v

•

11:14 min

April 14, 2015

•

5 Views

Balakrishnan S. Moorthy*¹, Lavanya K. Iyer*¹, Elizabeth M. Topp¹

¹Department of Industrial and Physical Pharmacy,Purdue University

概要

Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.