Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

Beomseok Seo; Junho Lee

doi:10.3791/54224

ジャーナル

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生物学

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蛍光を用いて観察し、テロメアの定量化と反復配列その場で</emPNAプローブのある>ハイブリダイゼーション（FISH）線虫（Caenorhabditis elegans）</em

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence <em>In Situ</em> Hybridization (FISH) with PNA Probes in <em>Caenorhabditis elegans</em>

JoVE Journal
生物学

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JoVE Journal 生物学

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

蛍光を用いて観察し、テロメアの定量化と反復配列その場で</emPNAプローブのある>ハイブリダイゼーション（FISH）線虫（Caenorhabditis elegans）</em

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10,274 Views

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10:01 min

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August 04, 2016

DOI:

10.3791/54224-v

DOI:

10.3791/54224-v

•

10:01 min

August 04, 2016

•

25 Views

Beomseok Seo¹, Junho Lee^1,2,3

¹Institute of Molecular Biology and Genetics (IMBG),Seoul National University, ²Department of Biological Sciences,Seoul National University, ³Department of Biophysics and Chemical Biology,Seoul National University

筆記録

概要

We report a concise procedure of fluorescence in situ hybridization (FISH) in the gonad and embryos of Caenorhabditis elegans for observing and quantifying repetitive sequences. We successfully observed and quantified two different repetitive sequences, telomere repeats and template of alternative lengthening of telomeres (TALT).