This protocol describes a net mold-based method to create three-dimensional scaffold-free cardiac tissues with satisfactory structural integrity and synchronous beating behavior.
Bai, Y., Yeung, E., Lui, C., Ong, C. S., Pitaktong, I., Huang, C., Inoue, T., Matsushita, H., Ma, C., Hibino, N. A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation. J. Vis. Exp. (138), e58252, doi:10.3791/58252 (2018).