Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Vera Filipa Monteiro-Cardoso; Romain Le Bars; Francesca Giordano

doi:10.3791/64750

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Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

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生物学

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JoVE Journal 生物学

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

1,164 Views

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08:27 min

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October 20, 2023

DOI:

10.3791/64750-v

DOI:

10.3791/64750-v

•

08:27 min

October 20, 2023

•

10 Views

Vera Filipa Monteiro-Cardoso^1,2, Romain Le Bars^1,3, Francesca Giordano^1,2

¹Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS,Université Paris-Saclay, ²Inserm U1280, ³Imagerie-Gif, Light Microscopy Facility, Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS,Université Paris-Saclay

概要

The need for new approaches to study membrane contact sites (MCSs) has grown due to increasing interest in studying these cellular structures and their components. Here, we present a protocol that integrates previously available microscopy technologies to identify and quantify intra-organelle and inter-organelle protein complexes that reside at MCSs.

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Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

概要

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