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Particulate Matter: Particles of any solid substance, generally under 30 microns in size, often noted as Pm30. There is special concern with Pm1 which can get down to Pulmonary alveoli and induce Macrophage activation and Phagocytosis leading to Foreign body reaction and Lung diseases.

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo

JoVE 59734

 Immunology and Infection

SEM Imaging of Biological Samples

JoVE 10492

Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut


A scanning electron microscope (SEM) is an instrument that uses an electron beam to nondestructively image and characterize conductive materials in a vacuum. As an analogy, an…

 Biomedical Engineering

Isolation of Fecal Bacteria from Water Samples by Filtration

JoVE 10213

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - Arizona University
Demonstrating Author: Luisa Ikner


The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events…

 Environmental Microbiology

The Respiratory System

JoVE 10881

The respiratory system is comprised of the organs that enable breathing. Air enters the nostrils and mouth, followed by the pharynx (throat) and larynx (voice box), which lead to the trachea (windpipe). In the thoracic cavity, the trachea splits into two bronchi that allow air to enter the lungs. The bronchi split into progressively smaller bronchioles and terminate in small groups of tiny sacs in the lungs called alveoli, where gas exchange occurs. Air is cleansed in the nasal cavity, but anything that passes those defenses or enters through the mouth can be caught in the lungs. The lungs produce mucus that traps foreign particles, and the bronchi and bronchioles are lined with cilia that beat mucus and debris upward toward the throat for disposal (i.e., swallowing). Smoking damages the cilia, making removal of the excess mucus produced by smoking more difficult. This is one of the reasons smokers are more susceptible to respiratory infections. The trachea is a 10-12 cm long tube located in front of the esophagus that allows air to enter and exit the lungs. Its C-shaped hyaline cartilage keeps the trachea open. When the smooth muscle of the trachea contracts, the diameter of the trachea decreases and exhaled air is pushed out with great force (e.g., coughing). In cases of damage to the throat or mouth that blocks breathing, a tracheostomy, a surgica

 Core: Circulatory and Pulmonary Systems

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education

JoVE 56945

 Cancer Research

Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice

1Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital - Harvard Medical School, 2Department of Respiratory Medicine, University of Cambridge - Addenbrooke's Hospital, 3Lung Transplant Program, Brigham and Women's Hospital - Harvard Medical School, 4COPD and IPF Programs, Lovelace Respiratory Research Institute

JoVE 52236

 Medicine

Injecting Gryllus bimaculatus Eggs

1Colby College, 2Division of Evolutionary Developmental Biology, National Institute for Basic Biology, 3Department of Biological Sciences, National University of Singapore, 4Department Biology and Department of Neuroscience, Bowdoin College, 5Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology, Harvard University

JoVE 59726

 Developmental Biology

Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment

1Research Institute McGill University Health Centre, 2Department of Biological Sciences, Université de Québec à Montréal, 3Department of Microbiology & Immunology, McGill University, 4Department of Medicine, Division of Infectious Diseases, McGill University, 5Centre de Recherche du Centre Hospitalier de l'Université de Montréal, 6Département de microbiologie, infectiologie et immunologie, Université de Montréal

JoVE 59427

 Immunology and Infection

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II, U.S. Food and Drug Administration

JoVE 58947

 Bioengineering

Automation of a Positron-emission Tomography (PET) Radiotracer Synthesis Protocol for Clinical Production

1SOFIE, 2Department of Molecular & Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles (UCLA), 3Ahmanson Translational Imaging Division, University of California, Los Angeles (UCLA), 4Crump Institute for Molecular Imaging, University of California, Los Angeles (UCLA)

JoVE 58428

 Chemistry

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Department of Chemistry, Skidmore College, 3Candiac MR Center, Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero

JoVE 59519

 Biochemistry

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

1Developmental and Stem Cell Biology, Hospital for Sick Children, 2Children's Health Research Institute, University of Western Ontario, 3Department of Physiology and Pharmacology, University of Western Ontario, 4Neurosciences and Mental Health, Hospital for Sick Children, 5Department of Paediatrics, University of Western Ontario

JoVE 51526

 Developmental Biology

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

1Department of Public Health, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

JoVE 58371

 Environment

Assessing the Particulate Matter Removal Abilities of Tree Leaves

1Key Laboratory of Silviculture and Conservation of Ministry of Education, Beijing Forestry University, 2School of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, Henan Normal University

JoVE 58026

 Environment

Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies

1Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 2Department of Environmental Sciences, College of the Coast & Environment, Louisiana State University, 3SCIREQ Scientific Respiratory Equipment Inc.

JoVE 58095

 Chemistry

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

1High Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences, Colorado State University, 2National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, 3Western Sydney University, 4Leprino Foods, Inc, 5Department of Food Science and Agricultural Chemistry, McGill University, 6Department of Mechanical Engineering, Colorado State University, 7Department of Animal Science, University of Wyoming

JoVE 58111

 Environment
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