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Pulsatile Flow: Rhythmic, intermittent propagation of a fluid through a Blood vessel or piping system, in contrast to constant, smooth propagation, which produces laminar flow.

Overview of Tissue Engineering

JoVE 5785

Tissue engineering is an emerging field, which aims to create artificial tissue from biomaterials, specific cells and growth factors. These engineered tissue constructs have far-reaching benefits, with possibilities for organ replacement and tissue repair.

This video introduces the field of tissue engineering and examines the components of engineered tissue. This video also outlines some prominent methods used to create the tissue scaffold, introduce a cell population and encourage growth and proliferation. Finally, some key challenges and important applications of the technology are demonstrated.


 Bioengineering

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

1Department of Bioengineering, The University of Texas at Arlington, 2Department of Medicine (Cardiology) and Bioengineering, UCLA, 3College of Health Science and Environmental Engineering, Shenzhen Technology University

JoVE 57763


 Bioengineering

Invasive Hemodynamic Characterization of the Portal-hypertensive Syndrome in Cirrhotic Rats

1Div. of Gastroenterology and Hepatology, Dept. of Internal Medicine III, Medical University of Vienna, Vienna, Austria, 2Vienna Hepatic Hemodynamic Laboratory, Medical University of Vienna, Vienna, Austria, 3Center of Biomedical Research, Medical University of Vienna, Vienna, Austria

JoVE 57261


 Medicine

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers

1Institute of Medical Science, University of Toronto, 2Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Department of Medicine, University of Toronto

JoVE 58478


 Bioengineering

Computational Fluid Dynamics Simulations of Blood Flow in a Cerebral Aneurysm

JoVE 10479

The objective of this video is to describe recent advancements of computational fluid dynamic (CFD) simulations based on patient- or animal-specific vasculature. Here, subject-based vessel segmentations were created, and, using a combination of open-source and commercial tools, a high-resolution numerical solution was determined within a flow model. Numerous studies have demonstrated that the hemodynamic conditions within the vasculature affect the development and progression of atherosclerosis, aneurysms, and other peripheral artery diseases; concomitantly, direct measurements of intraluminal pressure, wall shear stress (WSS), and particle residence time (PRT) are difficult to acquire in vivo. CFD allow such variables to be assessed non-invasively. In addition, CFD is used to simulate surgical techniques, which provides physicians better foresight regarding post-operative flow conditions. Two methods in magnetic resonance imaging (MRI), magnetic resonance angiography (MRA) with either time of flight (TOF-MRA) or contrast-enhanced MRA (CE-MRA) and phase-contrast (PC-MRI), allow us to obtain vessel geometries and time-resolved 3D velocity fields, respectively. TOF-MRA is based on the suppression of the signal from static tissue by repeated RF pulses that are applied to the imaged volume. A signal is obtained from un


 Biomedical Engineering

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

1Department of Surgery, Duke University Medical Center, 2Department of Biomedical Engineering, Duke University, 3School of Medicine, University of Pennsylvania, 4Department of Medicine, Division of Cardiology, Duke University Medical Center

JoVE 3349


 Bioengineering

Observational Study Protocol for Repeated Clinical Examination and Critical Care Ultrasonography Within the Simple Intensive Care Studies

1Department of Critical Care, University of Groningen, University Medical Centre Groningen, 2Department of Anesthesiology, University of Groningen, University Medical Centre Groningen, 3Department of Radiology, University of Groningen, University Medical Centre Groningen, 4Department of Cardiology, University of Groningen, University Medical Centre Groningen, 5Division of Nephrology, Department of Internal Medicine, University of Groningen, University Medical Centre Groningen

Video Coming Soon

JoVE 58802


 JoVE In-Press

Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro

1Department of Mechanical Engineering, University of California, Riverside, 2Division of Interventional Neuroradiology, University of California, Los Angeles, 3Materials Science and Engineering Program, University of California, Riverside, 4Department of Bioengineering, University of California, Riverside

Video Coming Soon

JoVE 58902


 JoVE In-Press

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, 2Department of Emergency Medical Services, Eulji University, 3Department of Mechanical Engineering, Sungkyunkwan University

Video Coming Soon

JoVE 58228


 JoVE In-Press

A Small Animal Model of Ex Vivo Normothermic Liver Perfusion

1Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Lab, Division of Transplant, Department of Surgery, Comprehensive Transplant Center, Ohio State University Wexner Medical Center, 2Department of Surgery, Division of Transplant, Ohio State University Wexner Medical Center, 3Department of Surgery, Division of CardioThoracic Surgery, Ohio State University Wexner Medical Center, 4Department of Medicine, Ohio State University Wexner Medical Center

JoVE 57541


 Medicine

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

1Transfusion Research Center, Belgian Red Cross-Flanders, 2Faculty of Medicine and Health Sciences, Ghent University, 3Blood Service, Belgian Red Cross-Flanders, 4Department of Public Health and Primary Care, KULeuven - University of Leuven

JoVE 55351


 Bioengineering

Near-infrared Navigation System for Real-time Visualization of Blood Flow in Vascular Grafts

1Department of Radiology, University of Missouri, 2Office of Animal Resources, University of Missouri, 3Bioengineering, University of Missouri, 4Biomedical Science, University of Missouri, 5Surgery - Division of Cardiothoracic Surgery, University of Missouri, 6MU-iCATS, University of Missouri, 7Medical Pharmacology and Physiology, University of Missouri

Video Coming Soon

JoVE 54927


 JoVE In-Press

Orthotopic Hind-Limb Transplantation in Rats

1Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, 2Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center

JoVE 2022


 Immunology and Infection

Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves

1Tissue Engineered Mechanics, Imaging and Materials Laboratory, Department of Biomedical Engineering, Florida International University, 2Department of Mechanical and Aerospace Engineering, University of Florida, 3College of Medicine, University of Florida, 4King Faisal Specialty Hospital and Research Center, Jeddah, Saudi Arabia

JoVE 50335


 Bioengineering

Adult Mouse Venous Hypertension Model: Common Carotid Artery to External Jugular Vein Anastomosis.

1Department of Anesthesia and Perioperative Care and Center for Cerebrovascular Research, University of California, San Francisco, 2Department of Neurological Surgery, University of California, San Francisco, 3Department of Neurology, University of California, San Francisco

JoVE 50472


 Medicine

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 2Division of Regenerative Medicine, Tulane National Primate Research Center, 3Department of Microbiology and Immunology, Tulane University School of Medicine, 4Department of Pharmacology, Tulane University School of Medicine

JoVE 50825


 Bioengineering

Increasing Pulmonary Artery Pulsatile Flow Improves Hypoxic Pulmonary Hypertension in Piglets

1Department of Medicine, Pulmonary Hypertension Research Group (CRIUCPQ), Laval University, 2Institut National de la Recherche Agronomique, 3Université Diderot Paris, Sorbonne Paris Cité, 4Hôpital Lariboisière, Physiologie clinique Explorations Fonctionnelles, 5INSERM U 965, 6Service de Cardiologie, Centre Hospitalier Universitaire Tours

JoVE 52571


 Medicine

Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System

1Department of Molecular Cell Biology, Leiden University Medical Center, 2Department of Engineering Technology, New Jersey Institute of Technology, 3Department of Urology, Leiden University Medical Center, 4Cardiovascular Research Institute, Department of Cell Biology and Molecular Medicine, Rutgers New Jersey Medical School

JoVE 52750


 Bioengineering

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