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Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements

1United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 2Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3Institute of Agriculture, Tokyo University of Agriculture and Technology

JoVE 53981


 Chemistry

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

1Bioenergy Research Unit, National Center for Agricultural Utilization Research, 2Mycotoxin Prevention and Applied Microbiology Research Unit, National Center for Agricultural Utilization Research, 3Chemical Engineering and Material Science, Great Lakes Bioenergy Center, Michigan State University

JoVE 54227


 Bioengineering

Tree Survey: Point-Centered Quarter Sampling Method

JoVE 10060

A number of methods are available for sampling forest communities. Point-centered quarter is one such method. It is used to gather information on the density, frequency, and coverage of tree species found in a forest. This information provides the ability to estimate the number of individual trees encountered, how often a certain tree occurs, how common the tree is compared to other trees, and the size of the tree. Compared to the standard plot analysis, the point-centered quarter method is more efficient, which is a major advantage. In a fixed-area plot sampling, a small portion of the total area of the forest is examined. In this small subsample, the density is determined directly by counting and identifying each tree. The ratio between size of the subplot and the overall forest size is used to determine the density for the entire forest.


 Environmental Science

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

1The School of Plant Sciences, University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, 3The Institute for Sustainable and Renewable Resources, The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences, Michigan State University

JoVE 51340


 Bioengineering

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

1Vegetable Crop Research Unit, USDA-ARS, 2Department of Entomology, University of Wisconsin-Madison, 3Department of Horticulture, University of Wisconsin-Madison, 4Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, 5Department of Bacteriology, University of Wisconsin-Madison, 6Laboratory of Genetics, Genome Center of Wisconsin, 7DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, 8J.F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison

JoVE 54631


 Environment

Determining the Mass Percent Composition in an Aqueous Solution

JoVE 10172

Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry

Determining the composition of a solution is an important analytical and forensic technique. When solutions are made with water, they are referred to as being aqueous, or containing water. The primary component of a solution is referred to as the solvent, and the dissolved minor component is called the solute. The solute is dissolved in the solvent to make a solution. Water is the most common solvent in everyday life, as well as nearly all biological systems. In chemistry labs, the solvent may be another liquid, like acetone, ether, or an alcohol. The solute can be a liquid or a solid, but this experiment only addressesthe determination of solids.


 General Chemistry

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography

1Department of Bioengineering, Stanford University, 2Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Umeå University, 3Campus de Cantoblanco, Universidad Autonoma de Madrid, 4Department of Microbiology and Immunology, Stanford University School of Medicine

JoVE 51183


 Chemistry

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

1Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), 2Faculty of Science and Technology, Free University of Bolzano, 3Department of Agriculture, Food and Environmental Sciences, University of Foggia, 4Institute of Analytical Chemistry & Radiochemistry, Leopold-Franzens Universität Innsbruck, 5Institut für Ionenphysik und Angewandte Physik, Leopold-Franzens Universität Innsbruck

JoVE 54075


 Chemistry

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva

1Department of Medicine, Weill Cornell Medical College, 2Department of Oral Biology, University of Missouri-Kansas City-School of Dentistry, 3Department of Pharmacology and Toxicology, University of Missouri Kansas City- School of Pharmacy, 4Regional Hospital, Bamenda, NWP, Cameroon, 5Mezam Polyclinic HIV/AIDS Treatment Center, Cameroon, 6Institute for Human Genetics and Biochemistry

JoVE 3999


 Medicine

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

1Department of Plant Biology, Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering, Stanford University, 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS), University of Freiburg

JoVE 4290


 Bioengineering

Purifying Compounds by Recrystallization

JoVE 10184

Source: Laboratory of Dr. Jimmy Franco - Merrimack College

Recrystallization is a technique used to purify solid compounds.1 Solids tend to be more soluble in hot liquids than in cold liquids. During recrystallization, an impure solid compound is dissolved in a hot liquid until the solution is saturated, and then the liquid is allowed to cool.2 The compound should then form relatively pure crystals. Ideally, any impurities that are present will remain in the solution and will not be incorporated into the growing crystals (Figure 1). The crystals can then be removed from the solution by filtration. Not all of the compound is recoverable — some will remain in the solution and will be lost. Recrystallization is not generally thought of as a separation technique; rather, it is a purification technique in which a small amount of an impurity is removed from a compound. However, if the solubility properties of two compounds are sufficiently different, recrystallization can be used to separate them, even if they are present in nearly equal amounts. Recrystallization works best when most impurities have already been removed by another method, such as extraction or column chromatography. Fi</span>…		</p>
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	<span title='Science Education: Essentials of Organic Chemistry' data-section-id='41' data-section-link='se' class='section_icon section_icon_se_orgchem  section_icon_link  section_icon_noaccess '> <span class='tooltip section_icon_tooltip'>Organic Chemistry <span title='Essentials of Organic Chemistry' data-section-id='41' data-section-link='se' class='section_icon section_icon_se_orgchem'></span></span></span></div>
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An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

1Center for Oral Biology, University of Rochester Medical Center, 2State Key Laboratory of Oral Diseases, Sichuan University, 3Department of General Medicine, Glostrup Hospital, Glostrup, Denmark, 4Department of Microbiology and Immunology, University of Rochester Medical Center

JoVE 2512


 Immunology and Infection

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