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Vas Deferens: The excretory duct of the testes that carries Spermatozoa. It rises from the Scrotum and joins the Seminal vesicles to form the ejaculatory duct.

Diagnostic Necropsy and Tissue Harvest

JoVE 10294

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of the samples. This manuscript will outline proper necropsy techniques for rats.


 Lab Animal Research

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London

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JoVE 56263


 JoVE In-Press

Sterile Tissue Harvest

JoVE 10298

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research.1 The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting tissues and organs for use in cell and tissue cultures requires aseptic technique to avoid contamination of the tissues. Sterile harvest is also necessary for protein and RNA analysis and metabolic profiling of tissues. This manuscript will discuss the process of sterile organ harvest in rats and mice.


 Lab Animal Research

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

1School of Life Science and Technology, ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

JoVE 55700


 Developmental Biology

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Ultrasound-guided Botulinum Toxin-A Injections: A Method of Treating Sialorrhea

1Clinical and Biological Sciences Department, Neurology Unit, University of Torino, San Luigi Gonzaga Hospital, 2Oncology Department, Radiology Unit, University of Torino, San Luigi Gonzaga Hospital, 3Clinical and Biological Sciences Department, Dietologic and Nutrition Unit, University of Torino, San Luigi Gonzaga Hospital

JoVE 54606


 Medicine

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Non-invasive Assessments of Subjective and Objective Recovery Characteristics Following an Exhaustive Jump Protocol

1Department of Business Economics, Health and Social Care, University of Applied Sciences and Arts of Southern Switzerland, 2University College Physiotherapy "Thim van der Laan", 3Department of Movement and Sport Sciences, Vrije Universiteit Brussel, 4Faculty of Medicine and Health Sciences, University of Antwerp

JoVE 55612


 Medicine

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The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

1Molecular and Cellular Physiology, Louisiana State University Health Sciences Center in Shreveport, 2Biological Sciences, Louisiana Tech University, 3Neurology, Louisiana State University Health Sciences Center in Shreveport, 4Institut Cochin, Inserm U1016, Cnrs Umr8104, Université Paris Descartes

JoVE 54632


 Biology

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MRI-guided dmPFC-rTMS as a Treatment for Treatment-resistant Major Depressive Disorder

1Institute of Medical Sciences, University of Toronto, 2MRI-Guided rTMS Clinic, University Health Network, 3Department of Psychiatry, University Health Network, 4Toronto Western Research Institute, University Health Network, 5Department of Psychiatry, University of Toronto, 6Faculty of Arts and Science, University of Toronto, 7Temerty Centre for Therapeutic Brain Intervention, Centre for Addiction and Mental Health

JoVE 53129


 Medicine

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Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

1Academic Geriatric Medicine, University of Southampton, University Hospital Southampton, 2National Institute for Health Research Southampton Biomedical Research Center, University of Southampton and University Hospital Southampton NHS Foundation Trust, 3MRC Lifecourse Epidemiology Unit, University of Southampton, 4National Institute for Health Research Musculoskeletal Biomedical Research Unit, University of Oxford, 5National Institute for Health Research Collaboration for Leadership in Applied Health Research and Care, 6Newcastle University Institute of Ageing and Institute of Health and Society, Newcastle University

JoVE 53075


 Medicine

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Murine Kidney Transplant Technique

1Colorado Center for Transplantation Care, Research and Education, University of Colorado, Denver, 2Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado, Denver, 3Department of Medicine, Division of Renal Diseases and Hypertension, Medical Center and University of Colorado, Denver, 4Department of Surgery, Division of Transplant Surgery, University of Colorado School of Medicine, University of Colorado-Denver, 5Renal Section, Denver Veterans Affairs Medical Center

JoVE 52848


 Medicine

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Stimulation of Notch Signaling in Mouse Osteoclast Precursors

1Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, 2Department of Biological Sciences, University of the Sciences, 3The Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 4Department of Biology, College of Science, Technology, Engineering, & Mathematics, Eastern Washington University

JoVE 55234


 Developmental Biology

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Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with the Loss of Peritoneal Integrity

1Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Greenville Hospital System, 2Department of Pathology, Duke University Health System, 3Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Duke University

JoVE 4313


 Medicine

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Electrode Positioning and Montage in Transcranial Direct Current Stimulation

1Headache & Orofacial Pain Effort (H.O.P.E.), Biologic & Material Sciences, School of Dentistry, University of Michigan, 2Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, 3Charité, University Medicine Berlin, 4Department of Biomedical Engineering, The City College of New York

JoVE 2744


 Neuroscience

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