In JoVE (3)
Other Publications (0)
Articles by Lei Wang in JoVE
Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells Ieng Lam Lei1, Lei Bu2, Zhong Wang1 1Cardiac Surgery, University of Michigan, 2Leon H Charney Division of Cardiology, New York University School of Medicine In this protocol, derivation of cardiac progenitor cells from both mouse and human embryonic stem cells will be illustrated. A major strategy in this protocol is to enrich cardiac progenitor cells with flow cytometry using fluorescent reporters engineered into the embryonic stem cell lines.
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons Ji-Yong Kang1, Daichi Kawaguchi2, Lei Wang3 1Department of Neuroscience, School of Medicine, Tufts University, 2Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 3Department of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California, San Francisco Here, a procedure to selectively activate a neuronal protein with a short pulse of light by genetically encoding a photo-reactive unnatural amino acid into a target neuronal protein expressed in neurons in culture or in vivo is presented.
Data Processing Methods for 3D Seismic Imaging of Subsurface Volcanoes: Applications to the Tarim Flood Basalt Lei Wang1, Wei Tian2, Yongmin Shi2 1College of Engineering, Peking University, 2School of Earth and Space Sciences, Peking University Three-dimensional (3D) reflection seismology is a powerful method for imaging subsurface volcanoes. By using industrial 3D seismological data from the Tarim Basin, we illustrate how to extract the sills and the conduits of subsurface volcanoes from seismic data cubes.