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Visualiseren van antigeen specifieke CD4 + T-cellen met behulp van MHC klasse II tetrameren

Article DOI: 10.3791/1167-v • 15:43 min • March 6th, 2009
March 6th, 2009 •
1, 2, 3, 2
1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute

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Deze procedure toont de zuivering en in vitro expansie van antigeen specifieke CD4 + T-cellen uit heel het perifere bloed en de visualisatie met behulp van MHC klasse II tetrameren. Tetrameren toestaan ​​dat de directe visualisatie van T-cellen met een enkel antigen specificiteit en gedefinieerd MHC klasse II beperking.

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Immunologie CD4 + T-cel MHC klasse II tetrameren perifeer bloed mononucleaire cellen in vitro expansie flowcytometrie
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