Encyclopedia of Experiments: Immunology
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Transcript
Take a platelet product into a riboflavin and UV illumination storage bag.
Introduce riboflavin molecules into the platelet product.
Inoculate the platelet product with clinically relevant bacteria, ensuring thorough mixing.
Retrieve a small volume of the sample and perform a serial dilution.
Spread each appropriate dilution on a suitable agar medium, and incubate it to measure the initial bacterial load.
Expose the platelet product to UV light.
Riboflavin is absorbed into the bacterial cell, where it interacts with the bacterial nucleic acid.
The UV light exposure leads to photodegradation of the riboflavin-nucleic acid complex, resulting in strand breakage. This irreversible damage inactivates the bacteria.
Platelets remain unaffected by the treatment due to the lack of nucleic acid.
Post-treatment, retrieve the platelet sample.
Repeat serial dilution and spread plate procedure to quantify the post-treatment bacterial load.
Compare initial and post-treatment bacterial loads to gauge the efficacy of riboflavin and UV light treatment.
