Inactivation of Pathogens <em>via</em> Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate

Chien-Wei Cheng; Shwu-Yuan Lee; Tang-Yu Chen; Jeu-Ming P. Yuann; Chi-Ming Chiu; Shiuh-Tsuen Huang; Ji-Yuan Liang

doi:10.3791/63531

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

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08:25 min

April 6, 2022

DOI: 10.3791/63531-v

Chien-Wei Cheng¹, Shwu-Yuan Lee², Tang-Yu Chen¹, Jeu-Ming P. Yuann¹, Chi-Ming Chiu¹, Shiuh-Tsuen Huang^3,4, Ji-Yuan Liang¹

¹Department of Biotechnology,Ming Chuan University, ²Department of Tourism and Leisure,Hsing Wu University, ³Department of Science Education and Application,National Taichung University of Education, ⁴Department of Soil and Environmental Sciences,National Chung Hsing University

Overview

This protocol describes the use of visible violet light to induce flavin mononucleotide photolysis, generating free radicals that can inhibit pathogenic bacteria such as Staphylococcus aureus and E.coli. The method is simple, safe, and suitable for various applications including therapy for injured skin and sanitation in the food industry.

Key Study Components

Area of Science

Microbiology
Phototherapy
Sanitation Techniques

Background

Flavin mononucleotide (FMN) acts as a photosensitizer.
Photolysis of FMN produces reactive oxygen species.
Visible light irradiation is safe and cost-effective.
Pathogenic bacteria pose significant health risks.

Purpose of Study

To develop a protocol for inactivating pathogenic bacteria.
To explore the efficacy of FMN under low-intensity light.
To provide a method applicable in clinical and industrial settings.

Methods Used

Visible violet light irradiation for FMN photolysis.
Testing against Staphylococcus aureus and E.coli.
Use of optical fibers for targeted illumination.
Evaluation of safety and effectiveness in various applications.

Main Results

FMN effectively generates free radicals under violet light.
Significant inhibition of pathogenic bacteria observed.
Method demonstrated safety for therapeutic use.
Potential applications in food sanitation confirmed.

Conclusions

FMN photolysis is a viable method for bacterial inactivation.
Visible light irradiation is a safe and effective approach.
This protocol can be adapted for various therapeutic and sanitation purposes.

Frequently Asked Questions

What is flavin mononucleotide?

Flavin mononucleotide (FMN) is a photosensitizer that can generate reactive oxygen species when exposed to light.

How does the protocol work?

The protocol uses violet light to induce FMN photolysis, producing free radicals that inhibit bacteria.

Is this method safe?

Yes, the method uses low-intensity light and is considered safe for therapeutic applications.

What bacteria were tested?

The protocol tested its efficacy against Staphylococcus aureus and E.coli.

Can this method be used in food sanitation?

Yes, the method is applicable for sanitation practices in the food industry.

What equipment is needed?

The method requires visible violet light sources and optical fibers for illumination.

Here, we present a protocol to inactivate pathogenic bacteria with reactive oxygen species produced during photolysis of flavin mononucleotide (FMN) under blue and violet light irradiation of low intensity. FMN photolysis is demonstrated to be a simple and safe method for sanitary processes.

This protocol uses visible violet light irradiation to induce flavin mononucleotide photolysis. This produces a large amount of free radicals, which inhibit pathogenic bacteria such as Staphylococcus aureus and E.coli. We have tested flavin mononucleotide as an appropriate photosensitizer.

In this protocol, we use visible violet light which is safe and doesn't require expensive equipment. This method can be used for the therapy of injured skin or infected subcutaneous tissues by inserting an optical fiber for illumination. It can also be applied to the sanitation practice in food industry.

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