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Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
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Bioengineering
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JoVE Journal Bioengineering
Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

DOI:
10.3791/63531-v

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08:25 min

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April 06, 2022

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, , , , , ,
1Department of Biotechnology,Ming Chuan University, 2Department of Tourism and Leisure,Hsing Wu University, 3Department of Science Education and Application,National Taichung University of Education, 4Department of Soil and Environmental Sciences,National Chung Hsing University

Chapters

  • 00:04Introduction
  • 01:21Photolysis System Setup and Detection of Superoxide Radicals
  • 03:11Viability of Staphylococcus aureus After FMN Photolysis
  • 05:03Detection of Superoxide Radicals in Staphylococcus aureus
  • 05:57Results: Effect of FMN Photolysis on Viability of Staphylococcus aureus
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to inactivate pathogenic bacteria with reactive oxygen species produced during photolysis of flavin mononucleotide (FMN) under blue and violet light irradiation of low intensity. FMN photolysis is demonstrated to be a simple and safe method for sanitary processes.

Tags

Keywords: PhotolysisRiboflavin-5′-phosphateVisible LightFree RadicalsStaphylococcus AureusE. ColiPhotosensitizerFood IndustrySanitationLEDSuperoxide RadicalsNBTFormazan

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