Encyclopedia of Experiments: Immunology
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Transcript
Begin with an antibody microarray chip printed with specific antibodies.
Each spot contains a unique antibody against a specific cyanobacterial strain.
Treat the chip with a blocking buffer to minimize non-specific interactions.
Assemble the chip in a hybridization cassette possessing multiple wells.
Introduce an environmental sample into the wells and incubate it under optimized conditions.
The sample contains various bacteria, including different cyanobacterial strains, that interact with specific antibodies on the printed chip. Wash the chip to remove the unbound bacteria.
Add an antibody mixture containing fluorophore-labeled anti-cyanobacterial-strain antibodies interacting with specific antigens on the respective cyanobacterial cells. Remove unbound antibodies.
Disassemble the cassette, wash and dry the chip, then scan it.
Fluorescence in specific spots signifies the presence of particular cyanobacterial strains in the sample.
