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Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
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JoVE Journal Chemistry
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

DOI:
10.3791/53644-v

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09:05 min

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January 06, 2016

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,
1Department of Chemistry,University at Albany, State University of New York, 2Multiplex Biotechnology Laboratory,Cancer Research Center

Chapters

  • 00:05Title
  • 00:44One-dimensional DNA Barcode Slides: Preparing the PDMS Barcode Mold
  • 02:21One-dimensional DNA Barcode Slides: Patterning of Poly-L-lysine (PLL)
  • 03:39One-dimensional DNA Barcode Slides: Patterning of ssDNA
  • 05:00Two-dimensional 3×3 DNA Arrays: Preparing the PDMS Barcode Mold and Flow Patterning of ssDNA
  • 06:17Two-dimensional 3×3 DNA Arrays: Validation and Immunoassay
  • 07:32Results: Multiplex-protein Detection from Single-cell Preparations
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines the fabrication of a large-scale, multiplexed two-dimensional DNA or antibody array, with potential applications in cell signaling studies and biomarker detection.

Tags

Flow-pattern Guided FabricationHigh-density Barcode Antibody MicroarraySingle-cell AnalysisDetect Secret ProteinsIntracellular ProteinsMicro-array PlatformCustomizable TechniqueFlow Patterning StrategiesSU-8 MasterChrome Photo-maskPhoto-resist CoatUV Pattern ApplicationPDMS Barcode Mold PreparationSilicon Elastomer BaseCuring AgentDe-gas Pre-polymer SolutionPetri DishSilicon WaferDepth Of 7.45 MillimetersDe-gas MixtureBaking At 75 Degrees Celsius To Cure PDMS

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