Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Erika S. Hue; Christine I. Fortier; Aur&#233;lie M. Laurent; Yann F. Quesnelle; Guillaume D. Fortier; Lo&#239;c J. Legrand; Stephane L. Pronost

doi:10.3791/53672

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면역학 및 감염병학

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호흡기 유체의 Equid 헤르페스 바이러스-2 진단에 대한 정량적 PCR 방법의 개발 및 검증

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

JoVE 신문
면역학 및 감염병학

This content is Free Access.

JoVE 신문 면역학 및 감염병학

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

호흡기 유체의 Equid 헤르페스 바이러스-2 진단에 대한 정량적 PCR 방법의 개발 및 검증

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28,306 Views

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09:57 min

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March 17, 2016

DOI:

10.3791/53672-v

DOI:

10.3791/53672-v

•

09:57 min

March 17, 2016

•

28264 Views

Erika S. Hue^1,2,3, Christine I. Fortier^1,3, Aurélie M. Laurent¹, Yann F. Quesnelle¹, Guillaume D. Fortier^1,2,3, Loïc J. Legrand^1,2,3, Stephane L. Pronost^1,2,3

¹LABÉO Frank Duncombe ²Unité de Risques Microbiens (U2RM, EA 4655),Normandy University ³Hippolia Foundation

Summary

Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.