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Immunology and Infection

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JoVE Journal Immunology and Infection
Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies
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Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Article DOI: 10.3791/54139-v • 11:10 min • June 29th, 2016
June 29th, 2016 •
1,2,3, 1,3,5, 1,2,3, 1,2,3, 1,2,3, 3,4, 1,2,3
1Department of Neurology, Mayo Clinic, 2Mayo Clinic Center for Multiple Sclerosis and Autoimmune Neurology, Mayo Clinic, 3Center for Regenerative Medicine, Neuroregeneration, Mayo Clinic, 4Division of Neonatal Medicine, Mayo Clinic, 5Department of Pediatric and Adolescent Medicine, Mayo Clinic

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요약
Automatic Translation

We present a protocol to identify protein moieties containing antigens for human and mouse IgM antibodies with specific kappa (Vκ) light chains. This protocol is not limited to IgM class antibodies but applies to all immunoglobulin isotypes that target their antigen with sufficiently high affinity during immunoprecipitations.

Tags

Antibody Binding Specificity Kappa Light Chain Human IgM Antibodies Polysialic Acid NCAM Case Study Therapeutic Potential Biomarkers Immunotherapeutic Agents Cancer Research Central Nervous System Neglected Class Of Antibodies Antibody-based Therapeutics Antigens Growing Tissue And Subcultures Biochemical Applications Robert Kahoud Jens Watzlawik Skull Cut Brain Extraction
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