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Journal / Genetics / Interrupción génica altamente eficiente de las…
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Genetics

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Interrupción génica altamente eficiente de las células progenitoras hematopoyéticas murinas y humanas por CRISPR/Cas9

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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Un protocolo para rápido CRISPR/Cas9-mediado interrupción de genes en ratón y células hematopoyéticas primarias humanas se describe en este artículo. Cas9 sgRNA ribonucleoproteínas se introducen a través de electroporación con sgRNAs generado a través de transcripción en vitro y Cas9 comercial. Alta eficiencia edición se logra con tiempo limitado y costos financieros.

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Genética número 134 CRISPR Cas9 HSPCs células madre y progenitoras hematopoyéticas knock-out ribonucleoproteína gene edición,
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