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Journal / Genetics / Högeffektiva gen störningar av murina och…
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Genetics

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JoVE Journal Genetics
Högeffektiva gen störningar av murina och mänskliga hematopoetiska stamceller av CRISPR/Cas9
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Högeffektiva gen störningar av murina och mänskliga hematopoetiska stamceller av CRISPR/Cas9

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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Ett protokoll för snabb CRISPR/Cas9-medierad gen störningar i mus och människans primära hematopoetiska celler är beskrivs i denna artikel. Cas9-sgRNA ribonucleoproteins introduceras via elektroporation med sgRNAs genereras genom in vitro- transkription och kommersiella Cas9. Hög redigering effektivitetsvinster uppnås med begränsad tid och finansiella kostnaden.

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Genetik fråga 134 CRISPR-Cas9 HSPCs hematopoetiska stamceller och stamceller celler knock-out ribonukleoprotein gen redigering,
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