Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Determination of Regulatory T Cell Subsets in…
JoVE Journal
Immunology and Infection

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

JoVE Journal Immunology and Infection
Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
Journal (Immunology and Infection)
A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
Journal (Immunology and Infection)
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
 

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

Article DOI: 10.3791/58848-v • 08:06 min • February 27th, 2019
February 27th, 2019 •
1, 1, 1, 1
1Department of Medical Cell Biology, Uppsala University

챕터

요약
Automatic Translation

Herein, we present a protocol to prepare single cells from murine thymus, pancreatic draining lymph node and spleen to further study these cells using flow cytometry. In addition, this protocol was used for determining the subsets of regulatory T cells using flow cytometry.

Tags

Regulatory T Cell Subsets Murine Thymus Pancreatic Draining Lymph Node Spleen Flow Cytometry Immune Cells Cost-effective Technique Manual Technique Zhengkang Luo PhD Student Euthanized Mice Scintillation Phials Conical Tubes Hank's Balanced Salt Solution RPMI-1640 Red Blood Cells Ammonium Chloride HBSS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter