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Immunology and Infection

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In Vitro ELISA test til vurdering af rabiesvaccine potens

Article DOI: 10.3791/59641-v • 09:04 min • May 11th, 2020
May 11th, 2020 •
1, 1,2
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

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Her beskriver vi en indirekte ELISA sandwich immunfangst til bestemmelse af det immunogene glycoprotein indhold i rabiesvacciner. Denne test bruger en neutraliserende Monoklonal Antistof (mAb-D1) genkende glycoprotein trimers. Det er et alternativ til in vivo NIH-testen for at følge konsistensen af vaccinestyrken under produktionen.

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Immunologi og infektion Problem 159 Rabiesvaccinestyrke NIH-test ELISA-metode mAb-D1 monoklonalt antistof Trimers af glycoprotein glycoproteinindhold
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