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Immunology and Infection

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In Vitro ELISA-test om de potentie van rabiësvaccin te evalueren

Article DOI: 10.3791/59641-v • 09:04 min • May 11th, 2020
May 11th, 2020 •
1, 1,2
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

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Hier beschrijven we een indirecte ELISA sandwich immunocapture om de immunogene glycoproproteïnegehalte in rabiësvaccins te bepalen. Deze test maakt gebruik van een neutraliserende Monoclonal Antibody (mAb-D1) herkennen glycoproproproteïne trimers. Het is een alternatief voor de in vivo NIH-test om de consistentie van de potentie van het vaccin tijdens de productie te volgen.

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Immunologie en infectie kwestie 159 rabiës vaccin potentie NIH test ELISA methode mAb-D1 monoklonale antilichaam Trimers van glycoproteïne Glycoprotein gehalte
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