Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Absolut kvantificering af celle frit protein…
JoVE Journal
Biochemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Biochemistry
Absolut kvantificering af celle frit protein syntese metabolisme ved omvendt fase-væskekromatografi-massespektrometri
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
Journal (Biochemistry)
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Journal (Biochemistry)
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
 
Click here for the English version

Absolut kvantificering af celle frit protein syntese metabolisme ved omvendt fase-væskekromatografi-massespektrometri

Article DOI: 10.3791/60329-v • 08:06 min • October 25th, 2019
October 25th, 2019 •
1, 1, *1, *1, 1
1Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University
* These authors contributed equally

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her præsenterer vi en robust protokol for at kvantificere 40 forbindelser involveret i Central Carbon og energimetabolisme i celle-fri proteinsyntese reaktioner. Den celle frie syntese blanding er derivatized med anilin for effektiv adskillelse ved hjælp af omvendt fase væskekromatografi og derefter kvantificeret ved massespektrometri ved hjælp af isotopisk mærkede interne standarder.

Tags

Biokemi celle-fri proteinsyntese væskekromatografi-massespektrometri anilin tagging central Carbon energimetabolisme intern standard metaboliske netværk isotop mærkning
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter