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Journal / Biochemistry / 通过反相液相-质谱法对无细胞蛋白合成代谢的绝对定量…
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Biochemistry

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JoVE Journal Biochemistry
通过反相液相-质谱法对无细胞蛋白合成代谢的绝对定量
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通过反相液相-质谱法对无细胞蛋白合成代谢的绝对定量

Article DOI: 10.3791/60329-v • 08:06 min • October 25th, 2019
October 25th, 2019 •
1, 1, *1, *1, 1
1Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University
* These authors contributed equally

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在这里，我们提出了一个强大的协议，以量化40个化合物参与中央碳和能量代谢在无细胞蛋白质合成反应。无细胞合成混合物用抗氧化剂衍生，使用反相液相色谱进行有效分离，然后使用异位标记的内部标准通过质谱法进行量化。

Tags

生物化学， 问题 152， 无细胞蛋白质合成， 液相色谱-质谱， 银氨酸标记， 中央碳， 能量代谢， 内部标准， 代谢网络， 同位素标签
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