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Measuring Photophysiology of Attached Stage of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer

Article DOI: 10.3791/63108-v • 07:03 min • November 12th, 2021
November 12th, 2021 •
1,2,3, 4, 1,2, 1
1Lake Biwa Branch Office, National Institute for Environmental Studies, 2Center for Regional Environmental Research, National Institute for Environmental Studies, 3Graduate School of Human Development and Environment, Kobe University, 4Lake Biwa Environmental Research Institute

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Fast repetition rate fluorometer (FRRf) is a beneficial method for measuring photosystem II photophysiology and primary productivity. Here we describe a protocol to measure PSII photophysiology of epizoic alga, Colacium sp. on substrate zooplankton using cuvette-type FRRf.

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Measuring Photophysiology Attached Stage Colacium Sp. Cuvette-type Fast Repetition Rate Fluorometer Photosynthetic Activity Real-time Measurement Sample Distraction Zooplankton Individuals Baseline Fluorescence Scapholeberis Mucronata FLW FWL Cuvette Optical Microscope Magnification Sample Volume Acclimate Low Light Act To Run F0 Value Colacium Species Molted Carapace Wash FLW Aseptically Inoculate AF-6 Medium Glass Tube
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