칸디다 알비칸스에서의 전사 인자-DNA 결합 상호작용의 게놈 전체 프로파일링: 포괄적인 CUT&RUN 방법 및 데이터 분석 워크플로우

Overview

This protocol outlines a method for conducting cleavage under targets and release using nuclease (CUT&RUN) in the human fungal pathogen Candida albicans. The protocol includes detailed experimental procedures and computational data analysis for profiling transcription factor-DNA binding interactions.

Key Study Components

Research Area

• Microbial genetics
• Transcription factor binding analysis
• Pathogen biology

Background

• Candida albicans as a human fungal pathogen
• Importance of studying transcription factor-DNA interactions
• The advantages of CUT&RUN over traditional methods

Methods Used

• Experiments involving epitope tagging and computational analysis
• Biological system: Candida albicans
• Technologies: CUT&RUN, fluorescence microscopy

Main Results

• CUT&RUN provides more accessible, sensitive, and high-quality data than ChIP-chip and ChIP-seq
• Successful profiling of Ndt80 and Efg1 binding events
• Identification of novel binding sites not previously reported

Conclusions

• The study demonstrates the efficiency of CUT&RUN for analyzing transcription factor interactions in pathogens
• Highlights the technique's relevance and potential adaptations to other forms of Candida albicans

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