Guest Editors
Alastair Barr
School of Life Sciences, University of Westminster, London
<p>Dr Alastair Barr is a Senior Lecturer in Pharmacology and Physiology in the School of Life Sciences, University of Westminster, London. His research is focussed on structural and functional studies of human receptor-type tyrosine phosphatases and their role in signalling pathways in health and disease. Prior to joining the University of Westminster he was a Team Leader at the Structural Genomics Consortium, Oxford and during this time carried out a large-scale structural analysis of the human phosphatome that was published in Cell. Dr Barr received his BSc (Hons) in Pharmacology from the University of Glasgow and a DPhil in Pharmacology from the University of Oxford. He carried out post-doctoral research at the University of Pennsylvania on G protein-coupled receptors (American Heart Association Fellowship), and at Duke University as an Assistant Research Professor working on chemoattractant receptor signalling in immune cells. He has also worked in the biotech and pharmaceutical sector.</p>
Michael Overduin
University of Alberta, Department of Biochemistry, Faculty of Medicine & Dentistry
<p>Dr. Michael Overduin is a Professor and CAIP Chair in the Department of Biochemistry at the University of Alberta, and Executive Director of NANUC, the national NMR centre in Edmonton. His lab focuses on structural biology and the discovery of ligands of proteins involved in cell adhesion, signaling and endocytosis. The overall aim is the development of mechanistic understanding of cancer progression and the structure-aided design of novel agents for therapeutic intervention. He focuses on mechanisms of desmosomal attachment to the cytoskeletion, phosphoinositide recognition by signaling proteins, and emerging targets including oncogenic phosphatases and kinases and the RhoA-Lbc complex. Methods including NMR are being used and developed to identify unexploited interdomain, lipid binding and allosteric sites, with the end goal of providing new avenues for intervention. Technological contributions include a computational method (“MODA”) which predicts lipid binding surfaces on protein structure, and the Styrene Maleic Acid Lipid Particle (SMALP) system for detergent-free purification of native membrane proteins into stable, soluble nanoparticles. </p>
Collection Overview
The study of protein-protein interactions is key to understanding the network of molecular interactions underlying biological effects, and defining how individual proteins perform their functional role within a cell. A detailed understanding of these interactions often provides an insight into aberrant signaling in disease. This collection brings together the wide range of techniques that have been developed to study interactions using in vitro, cell-based and in vivo approaches. Each technique has its own strengths, for example enabling systematic interrogation of the complete ‘interactome’, providing detailed atomic level information about a single interaction or insight into the sub-cellular localization of interacting proteins. Some techniques are easily adaptable to high-throughput screening of libraries or pulling out novel interacting partners while others have a more limited capacity. Readouts such as microscopy and mass-spectrometry are employed in protein-protein interaction techniques and some provide quantitative information on the affinity of an interaction.
Examples of methods to be included in this collection are, co-immunoprecipitation and antibody interference, pull-down assays using fusion proteins or modular protein domains, proximity-dependent labelling techniques (e.g. BioID), far western and receptor affinity probes, affinity purification coupled with mass-spectrometry, two-hybrid technologies (yeast and mammalian), fluorescence microscopy (e.g. immunofluoresence, FRET, super-resolution microscopy), protein complementation assays (e.g. BiFC), biophysical approaches (X-ray crystallography, NMR, AUC, SPR), and microarray based techniques (i.e. tissue and protein arrays).
Editorial
Articles
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
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Cited by 2
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2018
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Xiaofeng Zheng1,2, Calvin Qing Wei Ho1, Xiaowei Zheng3, Kian Leong Lee4, Katarina Gradin5, Teresa S. Pereira3, Per-Olof Berggren1,2,3, Yusuf Ali1,2
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute (SERI), Singapore General Hospital, 3The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, 4Cancer and Stem Cell Biology Program, Duke-NUS Medical School, 5Department of Cell and Molecular Biology, Karolinska Institutet
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
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Cited by 4
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2018
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Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
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Cited by 4
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2018
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1Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, 2Department of Biochemistry, University of Alberta, 3Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, 4DiscoveryLab, Faculty of Medicine & Dentistry, University of Alberta
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
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Cited by 14
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2020
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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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Cited by 6
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2020
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Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
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Cited by 4
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2021
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Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
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Cited by 2
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2022
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Nadezhda Podoplelova1,2, Polina Soloveva1,4, Andrei Garzon Dasgupta1,2,3, Aleksandra Filkova1,2, Mikhail Panteleev1,2,3,4
1Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics, Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics, Moscow Institute of Physics and Technology
Transmission Electron Microscopy: A Surgical Pathology Tool for Neuroblastoma
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Cited by 5
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2022
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Consolato M. Sergi*1,2,3,4, Janice Patry*1, Harry Coenraad1, Jeff McClintock1, Rod Nicholls1, Hans-Jörg Steiner5, Gregor Mikuz5
1Anatomic Pathology Division of EORLA, Children's Hospital of Eastern Ontario, University of Ottawa, 2Department of Laboratory Medicine and Pathology, University of Alberta, 3Department of Pediatrics, University of Alberta, Stollery Children's Hospital, 4Department of Orthopedics, Tianyou Hospital, Wuhan University of Science and Technology, 5Institute of Pathology, Medical University of Innsbruck
Abstracts
Native cell membrane nanoparticles system for protein-protein interaction analysis
Weihua Qiu1,
Kyle Kroeck2,
Youzhong Guo*1,
Claudio Catalano1
1Virginia Commonwealth University,
2Virginia Commonwealth University
Quantitative, double‐readout detection of protein-protein interactions in mammalian cells using LuTHY, a bioLuminescence‐based Two‐Hybrid technology
Philipp Trepte1,
Simona Kostova1,
Erich E. Wanker*1,
Sigrid Schnoegl1,
Christopher Secker1
1Max Delbrueck Center for Molecular Medicine in the Helmholtz Association
This is a title for a paper that I will not be submitting
test livetest*1
1JoVE
Microscale Thermophoresis: A fast and reliable biophysical tool to perform interaction analyses
Rahul Yadav1,
Deepak Kumar Saini*1,
Srivatsa Dwarakanath1,
Devendra Pratap Singh1
1Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science
The use of in situ Proximity Ligation Assay to detect potential interactions between survivin and Atg12-Atg5 conjugate for autophagy study
Chun Hei Antonio Cheung*1
1Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
<p>Protein Painting as a Method for Identification of Protein-Protein Interaction Sites</p>
Amanda Haymond*1
1GEORGE MASON UNIVERSITY
Isolation of neurospheres from neurogenic niches of adult prairie voles
Larry Young 1,
Raul Paredes2,
Nestor F Diaz*3,
Daniela Avila *4,
Wendy Portillo *2,
Francisco Camacho 5
12Silvio O Conte Center for Oxytocin and Social Cognition, Center for Translational Social Neuroscience, Yerkes National Primate Research Center, Department of Psychiatry and Behavioral Sciences, Emory University, Atlanta, Georgia 30329, United States of America,
2Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico ,
3Instituto Nacional de Perinatología, Isidro Espinosa de los Reyes, México,
4Instituto de Neurobiología, Universidad Nacional Autonoma de Mexico ,
5Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico
HEK293 and Expi293F based expression of glycoproteins used for membrane-protein interaction studies
Matthias Romauch*1
1Kar-Franzens-University, Graz
Unbiased Identification of Protein-Small Molecule Interactions by Isothermal Shift Assay (iTSA)
Steven Guard*1
1CU Boulder
A Method for the delivery of Foreign Material in the Murine Sciatic Nerve
Justin Tibbitts*1
1University of New Mexico
FRET as a tool for steady state interaction analyses of proteins in solution
Abhishek Garg1,
Athira P Janardhan1,
Deepak Kumar Saini*1,
Srivatsa Dwarakanath1,
Uchenna U. Waturuocha1
1Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science
<p>Isolation and culture of T lymphocytes from stenotic aortic valves using feeder cells from irradiated buffy coat.</p>
Lavinia Curini*1
1Charité Campus Benjamin Franklin