Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Methods Collections

Methods for unveiling mechanics in mechanobiology

COVID-19 response: Video production in affected areas is postponed, publishing text articles first

JoVE will publish text articles after the peer review, which on average takes two months after the manuscript submission. We will film and add corresponding videos to our website when laboratories and facilities in affected areas reopen.

Just Started
Methods Collection Image
Methods Collections
Methods for unveiling mechanics in mechanobiology

Guest Editors
Massimo Vassalli

University of Glasgow, Glasgow, UK

Dr. Massimo Vassalli is a Reader within the James Watt School of Engineering of the University of Glasgow and PI at the…

Mariana Azevedo Gonzalez Oliva

University of Glasgow, Glasgow, UK

Mariana Azevedo Gonzalez Oliva is currently a PhD student within the Centre for the Cellular Microenvironment (CeMi) at…

Giuseppe Ciccone

University of Glasgow, Glasgow, UK

Giuseppe Ciccone is a PhD student working with Dr. Massimo Vassalli in the field of mechanobiology within the Centre for…

Collection Overview

Cells continuously exert physical forces on their surrounding environment and simultaneously perceive and sustain the environment’s mechanical dynamics. This determines both cell and tissue mechanical properties and is reflected in physiological processes such as blood flow or muscle contraction, as well as in diseases such as cancer, where cell and microenvironment mechanics are notoriously altered.  

Deepening our understanding of this bidirectional crosstal­k requires new tools to apply, measure, and control cellular and microenvironmental mechanical properties, forces, and cellular mechanosensitivity. In this methods collection, we will collate novel approaches that have emerged within the field of mechanobiology and are ready to be standardised based on available reproducible commercial components. These will include techniques to quantify mechanical properties, including Brillouin spectroscopy, acoustic force spectroscopy for cell mechanics, and microfluidic technology for single cell mechanics. The collection will also include tools for quantifying cellular mechanosensation, such as high-specificity reporter dyes, specifically Ca2+ based mechanosensitivity assays and cellular and molecular force sensors, as well as high-throughput YAP/TAZ translocation assays.

We believe that this selection of complementary methods is central to highlight the crosstalk between mechanics and mechanosensitivity, and the importance of considering a variety of new approaches to delve into the future of mechanobiology.

Abstracts

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Isaac Li*1, Adam Yasunaga1, Vanessa White1, Seong Ho Kim1, Scott An1, Kathleen McMahon1, Yousif Murad1, Vojtěch Kapras1
1The University of British Columbia

Direct force measurements of subcellular mechanics in confinement using optical tweezers

Michael Krieg*1, Verena Ruprecht*2
1Neurophotonics and mechanical systems biology, ICFO, 2CRG

High-throughput Mechanobiology: Magnetic tweezers in a microplate

Christopher Toseland*1
1University of Sheffield

Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

Alyson Nguyen1, Keith Battle2, Sunita Pudel2, Ningyong Xu2, Jessica Bell3, Cassandra Chapman4, Ajay Singh5, Srinivas Palanki6, Thomas Rich7, Diego Alvarez8, Troy Stevens9, Dhananjay Tambe*10
1Biomedical Sciences, Pat Capps Covey College of Allied Health Professions, University of South Alabama, Mobile, Alabama, 2Department of Physiology and Cell Biology, College of Medicine, University of South Alabama, Mobile, Alabama, 3Center for Lung Biology, College of Medicine, University of South Alabama, Mobile, Alabama, 4William B. Burnsed Jr. Mechanical, Aerospace, and Biomedical Engineering Department, College of Engineering, University of South Alabama, Mobile, Alabama, 5Mitchell Cancer Institute, College of Medicine, University of South Alabama, Mobile, Alabama, 6Dan F. Smith Department of Chemical and Biomolecular Engineering, College of Engineering, Lamar University, Beaumont, Texas, 7Center for Lung Biology, Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, Alabama, 8Department of Physiology & Pharmacology, College of Osteopathic Medicine, Sam Houston State University, Conroe, Texas, 9Department of Physiology and Cell Biology, Center for Lung Biology, College of Medicine, University of South Alabama, Mobile, Alabama, 10Center for Lung Biology, Department of Pharmacology, College of Medicine, William B. Burnsed Jr. Mechanical, Aerospace, and Biomedical Engineering Department, College of Engineering, University of South Alabama, Mobile, Alabama

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter