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Pattern Generation for Micropattern Traction Microscopy
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Bioengineering
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JoVE Journal Bioengineering
Pattern Generation for Micropattern Traction Microscopy

Pattern Generation for Micropattern Traction Microscopy

DOI:
10.3791/63628-v

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09:26 min

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February 17, 2022

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, ,
1Department of Biomedical Engineering,Boston University

Chapters

  • 00:04Introduction
  • 00:30Subtractive Microcontact Printing
  • 04:16Polyacrylamide Gel Fabrication and Pattern Transfer
  • 08:01Results: An Improved Method of Indirectly Patterning PAA Hydrogels
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

We describe improvements to a standard method for measuring cellular traction forces, based on microcontact printing with a single subtractive patterning step of dot arrays of extracellular matrix proteins on soft hydrogels. This method allows for simpler and more consistent fabrication of island patterns, essential for controlling cell cluster shape.

Tags

Keywords: Pattern GenerationMicropattern Traction MicroscopyPDMSMaster MoldCoverslipsPlasma TreatmentFluorescent Labeled Protein3-APTMSGlutaraldehyde

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