Name: Microdissectie op meerdere niveaus en functioneel-structurele profilering van menselijke renale arteriële takken (Video) | JoVE Journal
Uploaded: 2025-09-05T13:00:00.000+00:00
Duration: 6 min 51 s
Description: Leer microdissectie en wire myografie voor functionele analyse van menselijke nierarteriën. Deze methode maakt directe vasculaire beoordeling mogelijk en verbetert translationeel geneesmiddelonderzoek.

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</ol>

<table><tbody><tr><td>Acetylcholine chloride</td><td>Sigma-Aldrich, Merck, Darmstadt, Germany</td><td>A6625</td><td></td></tr><tr><td>Kweekschaal met zwarte bodem</td><td>Danish Myo Technology, Aarhus, Denemarken</td><td>300412</td><td></td></tr><tr><td>CaCl2</td><td>Sangon Biotech Co.,Ltd.,Shanghai, China</td><td>A501330</td><td></td></tr><tr><td>D-glucose</td><td>Sangon Biotech Co.,Ltd.,Shanghai, China</td><td>A610219</td><td></td></tr><tr><td>Dumont pincet</td><td>Danish Myo Technology, Aarhus, Denemarken</td><td>300413</td><td></td></tr><tr><td>Geuder dissectieschaar</td><td>Danish Myo Technology, Aarhus, Denemarken</td><td>400431</td><td></td></tr><tr><td>Leidingdraad</td><td>Danish Myo Technology, Aarhus, Denemarken</td><td>400447</td><td>Diameter 40 &micro;m</td></tr><tr><td>KCl</td><td>Sangon Biotech Co.,Ltd.,Shanghai, China</td><td>A100395</td><td></td></tr><tr><td>KH2PO4</td><td>Sangon Biotech Co.,Ltd.,Shanghai, China</td><td>A100781</td><td></td></tr><tr><td>LabChart Professional versie 8.3&nbsp;</td><td>ADInstruments, Australië</td><td>-</td><td></td></tr><tr><td>MgCl2&middot;6H2O</td><td>Sangon Biotech Co.,Ltd.,Shanghai, China</td><td>A100288</td><td></td></tr><tr><td>Multi myograph systeem&nbsp;</td><td>Danish Myo Technology, Aarhus, Denemarken</td><td>620M</td><td></td></tr><tr><td>NaCl</td><td>Sangon Biotech Co.,Ltd.,Shanghai, China</td><td>A100241</td><td></td></tr><tr><td>NaHCO3</td><td>Sangon Biotech Co.,Ltd.,Shanghai, China</td><td>A100865</td><td></td></tr><tr><td>Fenylephrine</td><td>Sigma-Aldrich, Merck, Darmstadt, Germany</td><td>P6126</td><td></td></tr></tbody></table>

microdissectie op meerdere niveaus en functioneel-structurele profilering van menselijke renale arteriële takken

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Microdissectie op meerdere niveaus en functioneel-structurele profilering van menselijke renale arteriële takken

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Het gebruik van ultrahoge veld MRI als een niet-invasieve manier om fenotypische informatie te verkrijgen van knaagdiermodellen voor polycystische nierziekte en om interventies te monitoren, wordt beschreven. In vergelijking met de traditionele histologische aanpak, kunnen MRI-beelden in vivo worden verkregen, wat longitudinale follow-up mogelijk maakt.

Het gebruik van ultra-hoge gebied MRI in kleine knaagdieren Modellen van Polycystic voor<em&gt; In Vivo</em&gt; Fenotypering and Drug Monitoring

Dit protocol beschrijft een methode voor het isoleren en cultiveren van metanefrische rudimenten uit muisembryo's.

Dissectie en Cultuur van Muis Embryonale Nier

Weefsel-engineered renale constructies bieden een oplossing voor het tekort aan organen en de schadelijke effecten van dialyse. Hier beschrijven we een protocol bij micro ontleden lymfkliertest nieren voor isolatie van cortico-Wallenberg segmenten. Deze segmenten zijn steiger-vrije cellulaire constructies, vorming van renale organoids ingeplant.

Microdissection van primaire renale weefsel segmenten en integratie met nieuwe steiger-vrije constructie technologie

Een gemodificeerd 2 nier 1 clip (2K1C) Goldblatt muismodel werd ontwikkeld met behulp van polyurethaan tubing om nierslagaderstenose te initiëren, waardoor een toename van renine-expressie en nierbeschadiging werd geïnduceerd. Hier beschrijven we een gedetailleerde procedure voor het voorbereiden en plaatsen van de manchet op de nierslagader om een reproduceerbaar en consistent 2K1C-muismodel te genereren.

Een gemodificeerd twee nieren Een Clip Muis Model van Renine Regulatie in nierslagader Stenose

We beschrijven een protocol voor lasermicrodissectie van subsegmenten van de menselijke nier, waaronder de glomerulus, proximale tubulus, dikke opgaande ledemaat, het verzamelen van kanaal en interstitium. Het RNA wordt vervolgens geïsoleerd uit de verkregen compartimenten en RNA-sequencing wordt uitgevoerd om wijzigingen in de transcriptomische handtekening binnen elk subsegment te bepalen.

Toepassing van lasermicrodissectie om regionale transcriptomics in menselijk nierweefsel te ontdekken

Het protocol beschrijft de isolatie, cultuur en profilering van endotheelcellen uit de menselijke mesenteriale slagader. Daarnaast wordt een methode aangeboden om de menselijke slagader voor te bereiden op ruimtelijke transcriptomics. Proteomics, transcriptomics en functionele assays kunnen worden uitgevoerd op geïsoleerde cellen. Dit protocol kan worden hergebruikt voor elke middelgrote of grote slagader.

Isolatie en profilering van menselijke primaire mesenteriale arteriële endotheelcellen op transcriptoomniveau

Hier presenteren we een protocol met behulp van 2-fotonmicroscopie in München Wistar Fromter-ratten met oppervlakteglomeruli om de effecten van langdurige ureterale obstructie op glomerulaire dynamiek en functie te kwantificeren.

2-fotonenmicroscopie gebruiken om de effecten van chronische unilaterale ureterale obstructie op glomerulaire processen te kwantificeren

Hier presenteren we een gedetailleerd protocol voor proteomische analyse van de hele nier, geïsoleerde corticale tubulus en medullaire proteomen. De studie vergelijkt ook regionale proteomen in een diabetisch muismodel en niet-diabetische muizen.

Vergelijkende proteomische analyse van hele nier-, medulla- en corticale tubuli bij diabetische pathogenese van nierbeschadiging bij muizen

Leer microdissectie en wire myografie voor functionele analyse van menselijke nierarteriën. Deze methode maakt directe vasculaire beoordeling mogelijk en verbetert translationeel geneesmiddelonderzoek.

Microdissectie op meerdere niveaus en functioneel-structurele profilering van menselijke renale arteriële takken

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