A Duplex Digital PCR Assay for Simultaneous Quantification of the <em>Enterococcus spp.</em> and the Human Fecal-associated HF183 Marker in Waters

Yiping Cao; Meredith R. Raith; John F. Griffith

doi:10.3791/53611

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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

A Duplex Digital PCR Assay for Simultaneous Quantification of the <em>Enterococcus spp.</em> and the Human Fecal-associated HF183 Marker in Waters

JoVE Journal
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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

9,761 Views

•

12:14 min

•

March 09, 2016

DOI:

10.3791/53611-v

DOI:

10.3791/53611-v

•

12:14 min

March 09, 2016

•

3 Views

Yiping Cao¹, Meredith R. Raith¹, John F. Griffith¹

¹Department of Microbiology,Southern California Costal Water Research Project Authority

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Podsumowanie

This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.

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Duplex Digital PCR Assay Enterococcus Spp.HF183 Marker Fecal Contamination Water Quality Monitoring QPCR Master Mix Droplet Generation Positive Controls No Template Controls (NTCs)

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