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April 17, 2018
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Here, we describe a chromatographic assay coupled with the ion mobility separation of peptide precursors followed by the high-resolution (~30,000) MS-detection of peptide fragments for the quantification of spiked peptide standards in a monoclonal antibody digest.
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Doneanu, C., Fang, J., Alelyunas, Y., Yu, Y. Q., Wrona, M., Chen, W. An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry. J. Vis. Exp. (134), e55325, doi:10.3791/55325 (2018).
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