Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Emily  A. Brown; Steven  C. Neier; Claudia Neuhauser; Adam  G. Schrum; Stephen  E.P. Smith

doi:10.3791/60029

Dziennik

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Biochemia

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Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

JoVE Journal
Biochemia

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JoVE Journal Biochemia

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

8,244 Views

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00:07 min

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August 21, 2019

DOI:

10.3791/60029-v

DOI:

10.3791/60029-v

•

00:07 min

August 21, 2019

•

1 Views

Emily A. Brown^1,2, Steven C. Neier^3,4, Claudia Neuhauser⁵, Adam G. Schrum^6,7,8, Stephen E.P. Smith^1,2,9

¹Center for Integrative Brain Research,Seattle Children’s Research Institute, ²Graduate Program in Neuroscience,University of Washington, ³Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine,Harvard Medical School, ⁴Broad Institute of Harvard and MIT, ⁵Department of Mathematics,University of Houston, ⁶Department of Molecular Microbiology and Immunology, School of Medicine,University of Missouri, ⁷Department of Surgery, School of Medicine,University of Missouri, ⁸Department Bioengineering, College of Engineering,University of Missouri, ⁹Department of Pediatrics,University of Washington

Transkrypcja

Podsumowanie

Quantitative Multiplex Immunoprecipitation (QMI) uses flow cytometry for sensitive detection of differences in the abundance of targeted protein-protein interactions between two samples. QMI can be performed using a small amount of biomaterial, does not require genetically engineered tags, and can be adapted for any previously defined protein interaction network.