Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in <em>C. elegans</em>

Dhaval S Patel; Giovanni Diana; Eugeni V. Entchev; Mei Zhan; Hang Lu; QueeLim Ch'ng

doi:10.3791/56292

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Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in <em>C. elegans</em>

JoVE Journal
Genetyka

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JoVE Journal Genetyka

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

7,985 Views

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09:23 min

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August 16, 2017

DOI:

10.3791/56292-v

DOI:

10.3791/56292-v

•

09:23 min

August 16, 2017

•

27 Views

Dhaval S Patel*¹, Giovanni Diana*¹, Eugeni V. Entchev¹, Mei Zhan^2,3,4, Hang Lu^2,3,4, QueeLim Ch’ng¹

¹Centre for Developmental Neurobiology,King’s College London, ²Interdisciplinary Bioengineering Graduate Program,Georgia Institute of Technology, ³Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology, ⁴School of Chemical & Biomolecular Engineering,Georgia Institute of Technology

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Podsumowanie

Here, we present a framework to relate broad-range dietary restriction to gene expression and lifespan. We describe protocols for broad-range dietary restriction and for quantitative imaging of gene expression under this paradigm. We further outline computational analyses to reveal underlying information processing features of the genetic circuits involved in food-sensing.