Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Mariliis Tark-Dame; Blaise Weber; Mara de Sain; Damar Tri Anggoro; Rechien Bader; Aimee Walmsley; Rurika Oka; Maike Stam

doi:10.3791/57295

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Genetyka

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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

JoVE Journal
Genetyka

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JoVE Journal Genetyka

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

12,438 Views

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12:08 min

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March 28, 2018

DOI:

10.3791/57295-v

DOI:

10.3791/57295-v

•

12:08 min

March 28, 2018

•

6 Views

Mariliis Tark-Dame¹, Blaise Weber¹, Mara de Sain¹, Damar Tri Anggoro¹, Rechien Bader¹, Aimee Walmsley¹, Rurika Oka¹, Maike Stam¹

¹Swammerdam Institute for Life Sciences,University of Amsterdam

Transkrypcja

Podsumowanie

Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.