8,703 Views
•
10:28 min
•
July 15, 2018
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
Contamination during the genomic sequencing of microscopic organisms remains a large problem. Here, we show a method to sequence the genome of a tardigrade from a single specimen with as little as 50 pg of genomic DNA without whole genome amplification to minimize the risk of contamination.
Powiązane Filmy
13:26
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
Powiązane Filmy
10494 Views
13:03
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Powiązane Filmy
10481 Views
10:35
Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos
Powiązane Filmy
19759 Views
12:57
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
Powiązane Filmy
11378 Views
07:55
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Powiązane Filmy
7940 Views
12:08
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Powiązane Filmy
4917 Views
11:36
An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
Powiązane Filmy
1916 Views
10:34
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Powiązane Filmy
22607 Views
09:49
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
Powiązane Filmy
10719 Views
08:19
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Powiązane Filmy
10113 Views
Read Article
Cytuj ten artykuł
Yoshida, Y., Konno, S., Nishino, R., Murai, Y., Tomita, M., Arakawa, K. Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen. J. Vis. Exp. (137), e57615, doi:10.3791/57615 (2018).
Copy