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August 21, 2019
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Here, we describe a step-by-step strategy for isolating small RNAs, enriching for microRNAs, and preparing samples for high-throughput sequencing. We then describe how to process sequence reads and align them to microRNAs, using open source tools.
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Course, M. M., Gudsnuk, K., Valdmanis, P. N. A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools. J. Vis. Exp. (150), e59901, doi:10.3791/59901 (2019).
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